Automated Spectrophotometric Heparin Assays

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automated kinetic spectrophotometric assays of enzyme activities of human cerebrospinal fluid: methods and reference values.

Optimum conditions with respect to pH and molarity of phosphate buffer, and the concentrations of substrates, coenzymes, and exogenous enzyme, were defined at 37#{176}C for activity of aspartate transaminase and lactate dehydrogenase of human cerebrospinal fluid (CSF), as measured by optical kinetic assays at 340 nm. The resulting methods, and a previously published procedure for malate dehydro...

متن کامل

Automated enzyme assays.

1 . Introduction 2. What is Automation? 3. Solution-Based, Absorption Spectrophotometric Methods 3.1. Specimen Identification 3.2. Specimen Preparation 3.3. Specimen Integrity 3.4. Pipeting Specimens 3.5. Temperature Control 3.6. Absorbance Accuracy 3.7. Wavelength Accuracy 3.8. Time Control 3.9. Cuvet Control 3.10. Control of Interferences 3.10.1. Keto Acids 3.10.2. Ammonium Ions 3.10.3. Glyce...

متن کامل

Novel diagnostic assays for heparin-induced thrombocytopenia.

Laboratory testing for heparin-induced thrombocytopenia (HIT) has important shortcomings. Immunoassays fail to discriminate platelet-activating from nonpathogenic antibodies. Specific functional assays are impracticable due to the need for platelets and radioisotope. We describe 2 assays that may overcome these limitations. The KKO-inhibition test (KKO-I) measures the effect of plasma on bindin...

متن کامل

Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics

Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH(•), (•)NO, and O2 (•-) radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and ...

متن کامل

Automated determination of heparin with chromogenic substrates.

Spectrophotometric heparin assays which are based on the catalytic effect of heparin on either the inactivation of thrombin or that of factor Xa by antithrombin III, were adapted for use in a laboratory batch analyzer. Optimal conditions were determined for assays using the chromogenic substrates Chromozym-Th and S-2238 with thrombin, and S-2222 with factor Xa. Inactivation of the clotting enzy...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Pathophysiology of Haemostasis and Thrombosis

سال: 1984

ISSN: 1424-8832,1424-8840

DOI: 10.1159/000215056